|
| Status |
Public on Dec 12, 2012 |
| Title |
hypo-norm_rep6 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
hypoxia-treated locusts
|
| Organism |
Locusta migratoria |
| Characteristics |
tissue: thoratic flight muscle
|
| Treatment protocol |
Simulated 4km altitude hypoxia
|
| Growth protocol |
30 centidegree, light:dark=14h:10h, wheat bran as food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
40 µg of total RNA were primed with 2.5 µl of 2 µg/µl oligo(dT) at 68°C for 5 min, then reversed transcribed at 42°C for 3 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dCTP, dGTP, with 25 µM dTTP, 25 µM aa-dUTP
|
| |
|
| Channel 2 |
| Source name |
locusts in normoxia
|
| Organism |
Locusta migratoria |
| Characteristics |
tissue: thoratic flight muscle
|
| Treatment protocol |
Simulated 4km altitude hypoxia
|
| Growth protocol |
30 centidegree, light:dark=14h:10h, wheat bran as food
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
Cy5
|
| Label protocol |
40 µg of total RNA were primed with 2.5 µl of 2 µg/µl oligo(dT) at 68°C for 5 min, then reversed transcribed at 42°C for 3 h in the presence of 400 U SuperScript II RTase (Invitrogen), and 100 µM each dATP, dCTP, dGTP, with 25 µM dTTP, 25 µM aa-dUTP
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer were added, and samples were applied to microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed sequentially.
|
| Scan protocol |
Following instructions of GenePix 4000B Array Scanner; extract intensity information using GenePix Pro 6
|
| Description |
biological replicate 6 of 6
|
| Data processing |
Background corrected and LOWESS normalized; R plus limma package
|
| |
|
| Submission date |
Nov 22, 2011 |
| Last update date |
Dec 12, 2012 |
| Contact name |
Dejian Zhao |
| E-mail(s) |
dejian.zhao@yale.edu
|
| Organization name |
Yale School of Medicine
|
| Department |
Genetics
|
| Street address |
333 Cedar St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
|
| Platform ID |
GPL14920 |
| Series (1) |
| GSE33898 |
Locust flight muscle: hypoxia-treated vs control |
|
