|
| Status |
Public on Jul 14, 2024 |
| Title |
pcFLAG-hSTYK1 expressed Huh7 cells 1 |
| Sample type |
RNA |
| |
|
| Source name |
pcFLAG-hSTYK1 expressed Huh7 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Huh7
cell type: hepatocellular carcinoma
treatment: pcFLAG-hSTYK1 expressed
|
| Treatment protocol |
Huh7 cells were transfected with pcFLAG or pcFLAG-hSTYK1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from Huh7 cells transfected with pcFLAG or pcFLAG-hSTYK1 using RNeasy kit (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT Amplification Kit Module 1(Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentaion, single-stranded cDNA were hybridized for 20 hours at 48 ℃ with HuGene2.1ST Array Strip (Affymetrix) and GeneChip Expression 3'-Amplification Reagents Hybridization Control kit (Affymetrix).
|
| Scan protocol |
The array was scanned using Imaging Station (Affymetrix) according to the manufacture's instructions.
|
| Description |
Gene expression data from pcFLAG-hSTYK1-expressed Huh7 cells
|
| Data processing |
Arrays were summarized and normalized with Transcriptome Analysis Console.
|
| |
|
| Submission date |
Jul 09, 2024 |
| Last update date |
Jul 14, 2024 |
| Contact name |
Yasunori Sugiyama |
| E-mail(s) |
sugiyama@ag.kagawa-u.ac.jp
|
| Organization name |
Kagawa University
|
| Department |
Department of Life Science
|
| Lab |
Animal cell biology
|
| Street address |
ikenobe 2393
|
| City |
Miki-cho |
| State/province |
kagawa |
| ZIP/Postal code |
761-0795 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17692 |
| Series (1) |
| GSE271804 |
Whole genome expression microarray analysis in hepatocellular carcinoma cell line, Huh7 cells, transfected with hSTYK1 |
|
