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Status |
Public on Jul 14, 2024 |
Title |
pcFLAG-hSTYK1 expressed Huh7 cells 1 |
Sample type |
RNA |
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Source name |
pcFLAG-hSTYK1 expressed Huh7 cells
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Organism |
Homo sapiens |
Characteristics |
cell line: Huh7 cell type: hepatocellular carcinoma treatment: pcFLAG-hSTYK1 expressed
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Treatment protocol |
Huh7 cells were transfected with pcFLAG or pcFLAG-hSTYK1
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated from Huh7 cells transfected with pcFLAG or pcFLAG-hSTYK1 using RNeasy kit (QIAGEN).
|
Label |
Biotin
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Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT Amplification Kit Module 1(Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
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Hybridization protocol |
Following fragmentaion, single-stranded cDNA were hybridized for 20 hours at 48 ℃ with HuGene2.1ST Array Strip (Affymetrix) and GeneChip Expression 3'-Amplification Reagents Hybridization Control kit (Affymetrix).
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Scan protocol |
The array was scanned using Imaging Station (Affymetrix) according to the manufacture's instructions.
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Description |
Gene expression data from pcFLAG-hSTYK1-expressed Huh7 cells
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Data processing |
Arrays were summarized and normalized with Transcriptome Analysis Console.
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Submission date |
Jul 09, 2024 |
Last update date |
Jul 14, 2024 |
Contact name |
Yasunori Sugiyama |
E-mail(s) |
sugiyama@ag.kagawa-u.ac.jp
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Organization name |
Kagawa University
|
Department |
Department of Life Science
|
Lab |
Animal cell biology
|
Street address |
ikenobe 2393
|
City |
Miki-cho |
State/province |
kagawa |
ZIP/Postal code |
761-0795 |
Country |
Japan |
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|
Platform ID |
GPL17692 |
Series (1) |
GSE271804 |
Whole genome expression microarray analysis in hepatocellular carcinoma cell line, Huh7 cells, transfected with hSTYK1 |
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