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Status |
Public on Jun 18, 2012 |
Title |
2C litter 2 |
Sample type |
SRA |
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Source name |
2C stage embryos
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Organism |
Mus musculus |
Characteristics |
genetic background: mixed strain with C57/Bl6J developmental stage: 2-cell embryo tissue: embryo crosses: LSD1 FL/FL mice (From Wang et al, Nature 446, 2007 ) were crossed with ZP3::Cre mice (Jax labs strain 003651) and backcrossed again to generate LSD1 FL/FL, ZP3Cre+ and - mice. FOr this study, only LSD1 FL/FL, ZP3::Cre negative females were used to produce oocytes. For 2C embryos, these females were crossed with wild type CB6 males
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Treatment protocol |
Litters of 2C embryos were collected at E1.5 and lysed in Prelude direct lysis buffer
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Growth protocol |
Mixed strain female mice were fertilized with C57/Bl6J mice
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Extracted molecule |
total RNA |
Extraction protocol |
Nugen Ovation RNA-Seq kit VII (for amplifying RNA) followed by Covaris fragmentation of cDNA, and library construction using Illumina mRNA sample prep kit starting with end repair
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
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Description |
RNA amplified using SPIA
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Data processing |
2C_2.wig; genome build: mm9 Bowtie alignments, 3 mismatches per alignment, up to 20 alignments per read, for comparison with oocytes single-end (left side reads) trimmed on 3' end to 72bp
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Submission date |
Nov 23, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Todd Macfarlan |
E-mail(s) |
macfarlan@salk.edu
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Organization name |
The Salk Institute
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Street address |
10010 North Torrey Pines
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
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Platform ID |
GPL13112 |
Series (2) |
GSE33921 |
RNA-Seq from two-cell (2C) stage embryos |
GSE33923 |
2C::tomato ES cells, 2-cell embryos and wild type oocytes |
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Relations |
SRA |
SRX109431 |
BioSample |
SAMN00760871 |
Supplementary file |
Size |
Download |
File type/resource |
GSM838741_2C_2.bed.gz |
104.4 Mb |
(ftp)(http) |
BED |
SRA Run Selector![Help](/coreweb/images/long_help4.gif) |
Raw data are available in SRA |
Processed data provided as supplementary file |
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