|
| Status |
Public on Aug 01, 2012 |
| Title |
NA18502_HapMap |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
human_B cell line
|
| Organism |
Homo sapiens |
| Characteristics |
commercial source of sample: Coriell
|
| Biomaterial provider |
Coriell
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA18502
|
| Growth protocol |
standard for B cell lines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
standard DNA extraction from B cell lines using cell lysis
|
| Label |
Cy5
|
| Label protocol |
Agilent recommended protocol
|
| |
|
| Channel 2 |
| Source name |
human_reference_B cell line
|
| Organism |
Homo sapiens |
| Characteristics |
reference: NA10851_human_HapMap
|
| Biomaterial provider |
Coriell
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA10851
|
| Growth protocol |
standard for B cell lines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
standard DNA extraction from B cell lines using cell lysis
|
| Label |
Cy3
|
| Label protocol |
Agilent recommended protocol
|
| |
|
| |
| Hybridization protocol |
Agilent recommended protocol
|
| Scan protocol |
scan at 3 uM resolution
|
| Data processing |
Agilent Feature Extraction software. Dye bias correction was performed using default settings in Agilent Feature Extraction. The Log2 ratios were calculated using the ratio of rProcessedSignal to gProcessedSignal from the Feature Extraction files.
|
| |
|
| Submission date |
Nov 27, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Charles Lee |
| E-mail(s) |
clee@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital/Harvard Medical School
|
| Department |
Pathology
|
| Lab |
Lee Lab--Molecular Genetic Research Unit
|
| Street address |
221 Longwood Ave., EBRC-422
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL14930 |
| Series (1) |
| GSE33960 |
Regulatory element copy number differences shape primate expression profiles |
|
