|
| Status |
Public on Aug 01, 2012 |
| Title |
PR00724_Coriell |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
orangutan_B cell line
|
| Organism |
Pongo abelii |
| Characteristics |
commercial source of sample: Coriell
|
| Biomaterial provider |
Coriell
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=PR00724
|
| Growth protocol |
standard for B cell lines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
standard DNA extraction from B cell lines using cell lysis
|
| Label |
Cy5
|
| Label protocol |
Agilent recommended protocol
|
| |
|
| Channel 2 |
| Source name |
human_reference_B cell line
|
| Organism |
Homo sapiens |
| Characteristics |
reference: NA10851_human_HapMap
|
| Biomaterial provider |
Coriell
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=NA10851
|
| Growth protocol |
standard for B cell lines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
standard DNA extraction from B cell lines using cell lysis
|
| Label |
Cy3
|
| Label protocol |
Agilent recommended protocol
|
| |
|
| |
| Hybridization protocol |
Agilent recommended protocol
|
| Scan protocol |
scan at 3 uM resolution
|
| Data processing |
Agilent Feature Extraction software. Dye bias correction was peformed using a subset of probes with 100% sequence identity across human, chimpanzee, gorilla, orangutan, and rhesus macaque reference genomes. The Log2 ratios were calculated using the ratio of rProcessedSignal to gProcessedSignal from the Feature Extraction files.
|
| |
|
| Submission date |
Nov 27, 2011 |
| Last update date |
Aug 01, 2012 |
| Contact name |
Charles Lee |
| E-mail(s) |
clee@rics.bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital/Harvard Medical School
|
| Department |
Pathology
|
| Lab |
Lee Lab--Molecular Genetic Research Unit
|
| Street address |
221 Longwood Ave., EBRC-422
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL14930 |
| Series (1) |
| GSE33960 |
Regulatory element copy number differences shape primate expression profiles |
|
