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Sample GSM840533 Query DataSets for GSM840533
Status Public on Feb 10, 2017
Title Blood_Bo_C3_0W_rep3
Sample type RNA
 
Source name Peripheral Blood from Cattle before infected with schistosoma japonicum
Organism Bos taurus
Characteristics infection time: 0w
tissue: whole blood
Treatment protocol Cattle and buffalo were infected with cercariae by back patch.Before infection and after infection with 49d ,we collected peripheral blood from tha animals, and treated with TRIZOL Reagent as quickly as possible.
Growth protocol Peripheral blood were collected from the jugular vein of cattle,buffalo,1-2 year old,healthy,were kept in non-infected areas.And cercariae were provided by Jiangsu Institute of Schistosomiasis Control
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using TRIZOL Reagent (Cat#15596-018,Life technologies, Carlsbad, CA, US)following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).Qualified total RNA was further purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, One-Color (Cat#5190-2305, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany).
 
Hybridization protocol Each Slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat#5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat#G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat#121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat#5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions.
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings,Dye channel: Green ,Scan resolution=5μm, PMT 100%,10% ,16bit.
Description Gene expression profile of peripheral blood from cattle before infected with scistosoma japonicum, replicate3
Data processing The scanned images were analyzed with Feature Extraction software (Agilent technologies, Santa Clara, CA, US)with default settings.
 
Submission date Nov 29, 2011
Last update date Feb 10, 2017
Contact name yang jian mei
Organization name shanghai veterinary research institute
Street address minhang district ziyue road No.518
City shanghai
ZIP/Postal code 200241
Country China
 
Platform ID GPL11648
Series (1)
GSE34021 Comparison and analysis of gene expression between cattle and buffalo after infection with schistosoma japonicum

Data table header descriptions
ID_REF
VALUE processed Cy3 signal intensity (Agilent gProcessedSignal)

Data table
ID_REF VALUE
1 5.682512e+004
2 1.115091e+000
3 1.122006e+000
4 1.128468e+000
5 1.134673e+000
6 1.140539e+000
7 1.146046e+000
8 1.151356e+000
9 1.156277e+000
10 1.161010e+000
11 1.165399e+000
12 3.508567e+002
13 3.031063e+001
14 1.177193e+000
15 1.564358e+002
16 1.183943e+000
17 1.186897e+000
18 9.351536e+001
19 1.192516e+000
20 1.195098e+000

Total number of rows: 45220

Table truncated, full table size 872 Kbytes.




Supplementary file Size Download File type/resource
GSM840533_C3_0w_252364710150_S01_GE1_107_Sep09_1_3.txt.gz 2.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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