|
| Status |
Public on Dec 01, 2011 |
| Title |
CAF rep3 |
| Sample type |
RNA |
| |
|
| Source name |
CAF
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: colon cancer stage II
cell type: carcinoma associated fibroblast
|
| Growth protocol |
DMEM, 10% FCS, 1% ITS, 1% nonessential amino acids (Invitrogen), and 1% penicillin-streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was Extracted with Ambion's RNAqueous (Cat# 1911) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with Enzo's BioArray Synthesis Kit (PN 42406-10) from >7 ug total RNA .
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on HG-U133_Plus_2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner G3000.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Nov 30, 2011 |
| Last update date |
Dec 01, 2011 |
| Contact name |
Celia Chao |
| E-mail(s) |
cechao@utmb.edu
|
| Phone |
409-772-0698
|
| Organization name |
Univ TX Medical Branch
|
| Department |
Surgery
|
| Street address |
301 University Boulevard
|
| City |
Galveston |
| State/province |
TX |
| ZIP/Postal code |
77555-0737 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE34053 |
CD133+ colon cancer cells are more interactive with the tumor microenvironment than CD133- cells |
|
