|
| Status |
Public on Jul 26, 2024 |
| Title |
B35T1NC2 [Micro-C] |
| Sample type |
SRA |
| |
|
| Source name |
bronchial epithelium
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bronchial epithelium
cell line: B35T1NC
cell type: B35T1NC
|
| Growth protocol |
The cells were cultured in serum-free LHC-8 medium (Gibco, USA) at 37°C in an atmosphere containing 95% air and 5% CO2. The culture medium was changed every 3 days. When cells reached 90% confluency, they were detached by using trypsin (Sigma-Aldrich, USA) for 5 min, and the suspension was separated by centrifugation to collect the cells. The cells were then subjected to subculturing.
|
| Extracted molecule |
other |
| Extraction protocol |
DNA was extracted, purified and amplified with TruePrepTM Index Kit V2 for Illumina® (Vazyme)
Libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified by real-time PCR.Finally, DNA libraries were sequenced on the Illumina Novaseq platform and 150 bp paired-end reads were generated.
|
| |
|
| Library strategy |
OTHER |
| Library source |
other |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Micro-C data was processed using dovetail-genomics pipeline https://micro-c.readthedocs.io/en/latest/index.html
Assembly: hg19
Supplementary files format and content: hic
|
| |
|
| Submission date |
Jul 22, 2024 |
| Last update date |
Jul 26, 2024 |
| Contact name |
Yi Hu |
| E-mail(s) |
huyi301zlxb@sina.com
|
| Organization name |
Chinese PLA General Hospital
|
| Street address |
No. 28 Fuxing Rd， Haidian district，Beijing
|
| City |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE175860 |
Multi-omics datasets for finer-scale dissection of chromatin architecture alterations in radiation induced malignant transformation of bronchial epithelial cells. |
| GSE272820 |
Multi-omics datasets for finer-scale dissection of chromatin architecture alterations in radiation induced malignant transformation of bronchial epithelial cells [Micro-C] |
|
| Relations |
| BioSample |
SAMN42744614 |
| SRA |
SRX25416336 |
