|
| Status |
Public on Jul 13, 2012 |
| Title |
DAOY-MXD3 stable 2 |
| Sample type |
RNA |
| |
|
| Source name |
DAOY cells_transgenic_MXD3
|
| Organism |
Homo sapiens |
| Characteristics |
cell line background: DAOY
cell type: medulloblastoma
genotype/variation: MXD3 stable transgenic
transgene: pHM6-MXD3
|
| Growth protocol |
Cells were grown according to ATCC guidelines. Stable cell lines were selected and maintained in standard medium with 800 µg/ml G418.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen Rneasy kit according to manufacturer's instruction. RNA quality was checked with Agilent Bioanalizer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
SAMPLE 2
|
| Data processing |
Data was average-normalized and background substracted using Illumina BeadStudio.
|
| |
|
| Submission date |
Dec 02, 2011 |
| Last update date |
Jul 13, 2012 |
| Contact name |
Gustavo A. Barisone |
| E-mail(s) |
gabarisone@ucdavis.edu
|
| Organization name |
University of California Davis Health System
|
| Department |
Internal Medicine
|
| Street address |
4645 2nd. Ave
|
| City |
Sacramento |
| State/province |
CA |
| ZIP/Postal code |
95817 |
| Country |
USA |
| |
|
| Platform ID |
GPL6104 |
| Series (2) |
| GSE34100 |
Expression profiling of MXD3 stable cell lines |
| GSE34101 |
Role of MXD3 in DAOY cell proliferation |
|
