|
| Status |
Public on May 29, 2012 |
| Title |
immature Macrophages_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
monocyte derived macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: macrophages
infection: noninfected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAzol Lysis Reagent using the miRNeasy Mini Kit according to the manufacturer´s protocol (Qiagen, Hilden, Germany). Quality control was performed with denaturing gel electrophoresis and UV measurement.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared with the TargetAmp Nano-g Biotin aRNA Labeling Kit (Biozym Scientific GmbH, Hessisch Oldendorf, Germany).
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Immature macrophages
|
| Data processing |
The data were normalised using quantile normalization
|
| |
|
| Submission date |
Dec 02, 2011 |
| Last update date |
May 29, 2012 |
| Contact name |
Andrea Nino Castro |
| E-mail(s) |
anino@uni-bonn.de
|
| Organization name |
Life and Medical Sciences institute
|
| Lab |
Genomics and Immunoregulation
|
| Street address |
Carl Troll str. 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6884 |
| Series (1) |
| GSE34103 |
Genome wide analysis of macrophage gene expression after L. monocytogenes infection |
|
