|
Status |
Public on May 29, 2012 |
Title |
immature Macrophages_rep5 |
Sample type |
RNA |
|
|
Source name |
monocyte derived macrophages
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood cell type: macrophages infection: noninfected
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with QIAzol Lysis Reagent using the miRNeasy Mini Kit according to the manufacturer´s protocol (Qiagen, Hilden, Germany). Quality control was performed with denaturing gel electrophoresis and UV measurement.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA was prepared with the TargetAmp Nano-g Biotin aRNA Labeling Kit (Biozym Scientific GmbH, Hessisch Oldendorf, Germany).
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Immature macrophages
|
Data processing |
The data were normalised using quantile normalization
|
|
|
Submission date |
Dec 02, 2011 |
Last update date |
May 29, 2012 |
Contact name |
Andrea Nino Castro |
E-mail(s) |
anino@uni-bonn.de
|
Organization name |
Life and Medical Sciences institute
|
Lab |
Genomics and Immunoregulation
|
Street address |
Carl Troll str. 31
|
City |
Bonn |
State/province |
NRW |
ZIP/Postal code |
53115 |
Country |
Germany |
|
|
Platform ID |
GPL6884 |
Series (1) |
GSE34103 |
Genome wide analysis of macrophage gene expression after L. monocytogenes infection |
|