|
| Status |
Public on Jul 31, 2024 |
| Title |
C10WT Dele + gastrocnemius at P28, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C10WT Dele +
|
| Organism |
Mus musculus |
| Characteristics |
age: P28
tissue: gastrocnemius
genotype: C10WT Dele +
|
| Treatment protocol |
not applicable
|
| Growth protocol |
Mice were maintained on a 12-hour light/dark cycle with normal chow. Mouse gastrocnemius dissected from postnatal day 28 mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were genotyped beforehand. All samples were snap frozen in liquid N2. Total RNA were extracted from the frozen samples using Direct-zol RNA Miniprep Kit (Zymo, catalog R2051) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were enzymatically fragmented and biotinylated using the WT Terminal Labeling Kit (Applied Biosystems) according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Samples were hybridized using Hybridization Control Kit (Applied Biosystems) according to manufacturer's instructions.
|
| Scan protocol |
not provided
|
| Description |
gastrocnemius from mouse ID#5363
|
| Data processing |
Raw data was processed with Transcriptome Analysis Console (TAC v4.0.3) using the default settings, which uses a one-way ANOVA corrected with the Benjamini-Hochberg procedure for multiple procedures..
|
| |
|
| Submission date |
Jul 26, 2024 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Narendra Derek |
| E-mail(s) |
derek.narendra@nih.gov
|
| Phone |
301-594-4737
|
| Organization name |
National Institutes of Health
|
| Department |
National Institute of Neurological Disorders and Stroke
|
| Lab |
Inherited Movement Disorders Unit, Neurogenetics Branch
|
| Street address |
35 Convent Drive, Room 2A215
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20814 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE273193 |
Expression data from P28 C10 G58R Dele1 mouse gastrocnemius muscles. |
|
