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| Status |
Public on Aug 12, 2024 |
| Title |
cervical, control, rep2 |
| Sample type |
SRA |
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| Source name |
cervical
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| Organism |
Mus musculus |
| Characteristics |
tissue: cervical
cell type: Motor neuron
genotype: control
treatment: No treatment
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the PicoPure RNA isolation system.
rRNA depleted libraries were prepared from 10 to 20 ng of total RNA using the KAPA stranded RNA-seq kit with Riboerase (KAPA, #KK8483) and amplified by 15 cycles of PCR.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
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| Description |
rRNA-depleted
AV_061218_9
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| Data processing |
75 bp raw reads were analyzed by FASTQC (v0.11.9) for quality control.
aligned to mm39 genome assembly via STAR (v2.7.5b) aligner.
features were quantitated using featureCounts(v2.0.1).
Assembly: mm39
Supplementary files format and content: tab-delimited txt file contains raw counts for each sample
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| Submission date |
Jul 29, 2024 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Polyxeni Philippidou |
| E-mail(s) |
pxp282@case.edu
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| Organization name |
Case Western Reserve University
|
| Department |
Neuroscience
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| Lab |
Philippidou
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| Street address |
2210 Circle Dr.
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platform ID |
GPL17021 |
| Series (1) |
| GSE273364 |
Multimodal Hox5 activity generates motor neuron diversity [RNA-seq] |
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| Relations |
| BioSample |
SAMN42900909 |
| SRA |
SRX25508370 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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