|
| Status |
Public on Jun 07, 2012 |
| Title |
T47D_iEV_yesAP_noR5020_6h_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
iEV (inducible empty vector) cells treated with AP21967 and R5020 vehicle (ethanol) for 6 h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
genotype: PR null
parental cell line: T47D-Y
inducer treatment: AP21967
ligand treatment: vehicle
|
| Treatment protocol |
Cells were treated with 10e-9 M AP21967 durring the starvation with modified IMEM, and treated with 10e-8 M R5020 for 6 h
|
| Growth protocol |
T47D cells expressing inducible constructs were grown in minimal essential medium (5% FBS, 1% NEAA, 1% pennicilin/streptomycin, 6 ng/ml insulin, 200 ug/ml G418, 200 ug/ml hygromycin B) before being serum starved and AP21967 treated for 2 d, then treated with R5020
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A 2.0
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from T47D cells stably expressing inducible empty vectors, therefore are PR-null expressing cells, with dimerizer AP21967 treatment and no PR ligand treatment. Replicate 1.
|
| Data processing |
The data were analyzed with Bioconductor package simpleaffy in R using rma as normalization method.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Jun 10, 2012 |
| Contact name |
Todd P Knutson |
| E-mail(s) |
knut0297@umn.edu
|
| Phone |
612-626-8911
|
| Organization name |
University of Minnesota
|
| Department |
Minnesota Supercomputing Institute
|
| Street address |
117 Pleasant St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (2) |
| GSE34147 |
Phosphorylated and Sumoylation-Deficient Progesterone Receptors Drive Proliferative Gene Signatures During Breast Cancer Progression (Affymetrix gene expression analysis) |
| GSE34149 |
Phosphorylated and Sumoylation-Deficient Progesterone Receptors Drive Proliferative Gene Signatures During Breast Cancer Progression |
|
