|
| Status |
Public on Jun 07, 2012 |
| Title |
T47D_PR_KR_yesR5020_6h_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
T47D-Y cells expressing K388R PR treated with R5020 for 6 h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: breast
genotype: mutant K388R PR
parental cell line: T47D-Y
ligand treatment: R5020
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were log2 transformed and quantile normalized with Bioconductor lumi package in R.
|
| |
|
| Submission date |
Dec 05, 2011 |
| Last update date |
Feb 01, 2017 |
| Contact name |
Todd P Knutson |
| E-mail(s) |
knut0297@umn.edu
|
| Phone |
612-626-8911
|
| Organization name |
University of Minnesota
|
| Department |
Minnesota Supercomputing Institute
|
| Street address |
117 Pleasant St SE
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE34148 |
Phosphorylated and Sumoylation-Deficient Progesterone Receptors Drive Proliferative Gene Signatures During Breast Cancer Progression (Illumina gene expression analysis) |
| GSE34149 |
Phosphorylated and Sumoylation-Deficient Progesterone Receptors Drive Proliferative Gene Signatures During Breast Cancer Progression |
|
| Relations |
| Reanalyzed by |
GSM2474092 |
