|
| Status |
Public on Aug 14, 2024 |
| Title |
amniotic fluid fetal cells 4 |
| Sample type |
genomic |
| |
|
| Source name |
amniotic fluid fetal cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: amniotic fluid fetal cells
diagnosis: microdeletion of 22q11.21
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA of fetal cells in amniotic fluid or cord blood sampling was extracted with using the QIAamp DNA extraction kit (Qiagen, Dusseldorf, Germany)
|
| Label |
Biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
DNA was processed as follows. Restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer`s instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix Fluidics Stations, and scanned using the GeneChip Scanner 750K
|
| Description |
Hybridized to CytoScan 750K
|
| Data processing |
The array image was acquired using Affymetrix GeneChip® Command Console (AGCC version 4.0.0.1567). Data processing and analysis (CEL file generation, smoothing and segmentation) were used Chromosome Analysis Suite (version 3.3).
|
| |
|
| Submission date |
Aug 11, 2024 |
| Last update date |
Aug 14, 2024 |
| Contact name |
Yanting Que |
| E-mail(s) |
queyanting@outlook.com
|
| Organization name |
Fujian Medical University
|
| Street address |
No. 88, Jiaotong Road, Taijiang District, Fuzhou City, Fujian Province
|
| City |
Fuzhou |
| ZIP/Postal code |
350100 |
| Country |
China |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE274505 |
Copy number variation analysis of 18,000 fetuses in southern China |
|
