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| Status |
Public on Sep 20, 2024 |
| Title |
0d_s2 |
| Sample type |
SRA |
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| Source name |
cornea
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| Organism |
Macaca fascicularis |
| Characteristics |
tissue: cornea
genotype: WT
treatment: uninjured
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| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were filtered through a 30-µm cell strainer and were re-suspended in 60 µL PBS containing 0.04% BSA to achieve 1,000 cells µl-1 for capture on the 10× Genomics Chromium controller.
The dissociated single cells were processed on the GemCode Single Cell Platform per the manufacturer’s recommendations using the Chromium Single Cell 3′ GEM, Library, and Gel Bead Kit v3 (10× Genomics; PN-1000075) with a recovered quantity of ~2000 cells.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
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| Data processing |
Illumina library preparation was performed using the Chromium Single Cell 3′ Reagent Kits User Guide (V3 Chemistry). After the cDNA cleanup step (Step 2.1), half of the purified cDNA was used for PacBio library preparation, and the rest was used for downstream Illumina library preparation. Illumina libraries were sequenced on a NextSeq 550 system (SY-415-1002, Illumina) by using NextSeq High Output Kits (150 cycles; 20024907, Illumina) with the following read protocol: read 1, 118 cycles; i7 index read, 8 cycles; read 2, 40 cycles.
Assembly: Ensembl Macaca_fascicularis_5.0.99
Supplementary files format and content: Read count matrices for each sample were generated by CellRanger counting.
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| Submission date |
Sep 02, 2024 |
| Last update date |
Sep 20, 2024 |
| Contact name |
ming zhou |
| E-mail(s) |
zm2656581611@gmail.com
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| Phone |
13121694691
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| Organization name |
Sun Yat-sen University,
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| Street address |
jinsui road 7
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| City |
guangzhou |
| ZIP/Postal code |
510623 |
| Country |
China |
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| Platform ID |
GPL27448 |
| Series (1) |
| GSE276184 |
Single-cell transcriptomic analysis reveals dynamic cellular processes in corneal epithelium during wound healing in cynomolgus monkeys |
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| Relations |
| BioSample |
SAMN43462986 |
| SRA |
SRX25934252 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM8492742_0d_s2_barcodes.tsv.gz |
12.0 Kb |
(ftp)(http) |
TSV |
| GSM8492742_0d_s2_features.tsv.gz |
190.9 Kb |
(ftp)(http) |
TSV |
| GSM8492742_0d_s2_matrix.mtx.gz |
21.6 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
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