|
| Status |
Public on Apr 10, 2012 |
| Title |
HDBEC_8068.BEC.P3 |
| Sample type |
genomic |
| |
|
| Source name |
Dermal blood endothelial cells_buttock
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
age: 65 years
tissue: buttock
cell type: dermal blood endothelial cells
|
| Growth protocol |
Endothelial cells were grown in EGM-2 MV contained Endothelial Cell Basal Medium-2 (EBM-2) supplemented with 25 ml FBS, 0.2 ml hydrocortisone, 2 ml hFGF, 0.5 ml VEGF, 0.5 ml R3-IGF-1, 0.5
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted and purified from skin biopsy samples using QIAamp DNA Investigator Kit according to standard instructions
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite conversion and subsequent cleanup was carried out using EZ DNA Methylation™ Kit (D5001), Zymo Research; converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450 Beadchip using the standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using iScan Reader using standard recommended Illumina scanner setting
|
| Description |
punch biopsy
|
| Data processing |
BeadStudio software 2011.1 and R statistical software vers. 2.12.1 Non_normalized data matrix contains unmethylated (signal A) and methylated (signal B) signal intensities.
|
| |
|
| Submission date |
Dec 16, 2011 |
| Last update date |
Apr 10, 2012 |
| Contact name |
Silvia Rueberg |
| Organization name |
Miltenyi Biotec GmbH
|
| Department |
Genomic Services Department
|
| Street address |
Friedrich-Ebert-Str. 68
|
| City |
Bergisch Gladbach |
| ZIP/Postal code |
51429 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE34486 |
DNA methylation regulates lineage-specifying genes in the human vascular system [methylation array]. |
| GSE34487 |
DNA methylation regulates lineage-specifying genes in the human vascular system. |
|
