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| Status |
Public on Sep 20, 2024 |
| Title |
SN_CT rep3 |
| Sample type |
SRA |
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| Source name |
Substantia nigra
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| Organism |
Macaca fascicularis |
| Characteristics |
tissue: Substantia nigra
cell type: brain
treatment: CT
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| Extracted molecule |
total RNA |
| Extraction protocol |
The Cell nucleus in the Macaca_mulatta brain tissues were isolated according to manufacturer’s protocol(Nuclei PURE Prep,Sigma-Aldrich ).
The Cell nucleus were loaded onto the 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1,000 cells per μl (Single Cell 3′ library and Gel Bead Kit v.3) as described in the manufacturer’s protocol. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed as per the manufacturer’s protocol.Qubit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using the Cell Ranger software v7.0.1 (https://www.10xgenomics.com/cn/support/software/cell-ranger)
Assembly: hg38
Supplementary files format and content: Tab-separated values files and matrix files
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| Submission date |
Sep 09, 2024 |
| Last update date |
Sep 20, 2024 |
| Contact name |
wang yi chao |
| E-mail(s) |
mayiy3763@gmail.com
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| Phone |
13518740643
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| Organization name |
Kunming University of Science and Technology
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| Street address |
Jingming South Road, Chenggong District, Kunming City, Yunnan Province
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| City |
yunnan |
| State/province |
postgraduation |
| ZIP/Postal code |
650000 |
| Country |
China |
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| Platform ID |
GPL28212 |
| Series (1) |
| GSE276753 |
An accelerated Parkinson’s disease monkey model using AAV-α-synuclein plus Poly(ADP-ribose) |
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| Relations |
| BioSample |
SAMN43550330 |
| SRA |
SRX26029951 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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