|
| Status |
Public on Dec 20, 2011 |
| Title |
Haemonchus contortus adult |
| Sample type |
SRA |
| |
|
| Source name |
Whole organism
|
| Organism |
Haemonchus contortus |
| Characteristics |
strain: MHco3(ISE)
developmental stage: Mixed sex adult
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol (Invitrogen) and small RNA libraries were prepared using an Illumina Small RNA Sample Prep kit and the Alternative v1.5 Protocol.
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Mixed sex adult worms were collected at necropsy (day 28) from Suffolk lambs (5-9 months old) infected with 5,000 L3 larvae and maintained indoors to eliminate risk of other nematode infections.
|
| Data processing |
3984_2_WA_unique.txt; genome build: File "combined_worms_supercontigs200808.fasta" from ftp://ftp.sanger.ac.uk/pub/pathogens/Haemonchus/contortus/genome/contigs.
Processed files were produced from the raw data by trimming reads of adapter sequence, identifying identical reads and converting these to unique sequences with associated read counts, mapping sequences to the genome and retaining only those with perfect alignment.
|
| |
|
| Submission date |
Dec 19, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Alan Derek Winter |
| E-mail(s) |
Alan.Winter@glasgow.ac.uk
|
| Phone |
+44 141 330 5759
|
| Organization name |
University of Glasgow
|
| Department |
Institute of Infection, Immunity and Inflammation
|
| Street address |
Garscube Estate, Bearsden Road
|
| City |
Glasgow |
| ZIP/Postal code |
G61 1QH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL15032 |
| Series (1) |
| GSE34539 |
High-throughput sequencing of small RNAs from Brugia pahangi and Haemonchus contortus |
|
| Relations |
| SRA |
SRX112201 |
| BioSample |
SAMN00767517 |
