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Status |
Public on Dec 20, 2011 |
Title |
Porcine, control, biological, rep3 |
Sample type |
RNA |
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Source name |
Porcine alveolar Macrophages isolated from control pigs, 28 dpi
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Organism |
Sus scrofa |
Characteristics |
tissue: Alveolar Macrophage
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Treatment protocol |
Bronchoalveolar lavage of the lungs was performed with 100mL aliguots of sterile PBS containing genamicin at 70 μg/mL(Sigma).Then lavage fluids were centrifuged at 230 ×g for 15 min, and cells were washed twice with Dulbecco’s Modified Eagle’s Medium (DMEM) containing gentamicin (50μg/mL).
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Growth protocol |
Six 28 days old healthy pigs were obtained, Three pigs were inoculated intratracheally with 2 ml of 4.5×108 colony forming units of HPS4 strain MD0322. other three pigs were intratracheally challenged with 2 ml PBS as control. All pigs were slaughtered at 28 dpi.
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Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
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Description |
Gene expression data from Porcine alveolar Macrophages isolated from control pigs at 28 d.p.i.
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Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array
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Submission date |
Dec 19, 2011 |
Last update date |
Dec 20, 2011 |
Contact name |
Liu Shuqing |
E-mail(s) |
wentaovet@yahoo.com
|
Organization name |
Huazhong agricultural university
|
Street address |
No.1,Shizishan Street · Hongshan District
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City |
Wuhan |
State/province |
Hubei province |
ZIP/Postal code |
430070 |
Country |
China |
|
|
Platform ID |
GPL3533 |
Series (1) |
GSE34544 |
Expression data from Porcine alveolar macrophages of piglets experimentally infected with Haemophilus parasuis serotype 4 (HPS4) for 28 days |
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