|
| Status |
Public on Sep 25, 2024 |
| Title |
medium cultured with hWJ-MSCs_B alone |
| Sample type |
protein |
| |
|
| Source name |
Wharton’s jelly-derived human mesenchymal stem cell
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: medium cultured with hWJ-MSCs_B alone
|
| Treatment protocol |
We collected the conditioned media from the following groups to perform the array: C2C12 alone, hWJ-MSCs alone (A, B, C), hPL-MSCs alone (A, B, C), and C2C12 cells co-cultured with hWJ-MSCs (A, B, C), or hPL-MSCs (A, B, C).
|
| Growth protocol |
hWJ-MSCs and hPL-MSCs were maintained in MEM-a supplemented with 10% fetal bovine serum (FBS) and 0.5% gentamicin in humidifed atmosphere with 5% CO2 at 37℃. C2C12 were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 0.5% gentamicin in humidifed atmosphere with 5% CO2 at 37℃.
|
| Extracted molecule |
protein |
| Extraction protocol |
The secreted proteins in the conditioned media collected from hMSCs alone culture, C2C12 alone culture, and co-culture of hMSCs and C2C12 cells
|
| Label |
biotin
|
| Label protocol |
Sample was labeled according to the protocol in the kit manual from Raybiotech.
|
| |
|
| Hybridization protocol |
Drying the glass slide, Blocking and Incubations. Biotin labeled extract was hybridized with antibody-spotted slide for 2hr at room temperature. After hybridization, slide was washed with 1X wash buffer I and 1X wash buffer II and de-ionized water. Detection Buffer including Cy3-Streptavidin was treated for 2hr at room temperature in dark. And slide was washed with 1X wash buffer I, II and DW.
|
| Scan protocol |
Scanned on an GenePix 4100 scanner.
|
| Data processing |
GenePix Software (v 6.1) was used for data extraction.
|
| |
|
| Submission date |
Sep 13, 2024 |
| Last update date |
Sep 25, 2024 |
| Contact name |
Sun Jeong Kim |
| E-mail(s) |
xhxhfn78@gmail.com
|
| Phone |
82262058050
|
| Organization name |
ENCell Co. Ltd.
|
| Street address |
701, Yeongdong-daero, Gangnam-gu
|
| City |
Seoul |
| ZIP/Postal code |
06072 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL34899 |
| Series (1) |
| GSE277126 |
Anti-necroptotic effects of human Wharton’s jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-α |
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