|
| Status |
Public on Feb 10, 2012 |
| Title |
rrd2_root induction.12h.28C_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
rrd2 explant
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
strain: Landsberg erecta
genotype: rrd2 mutant
tissue: cultured explant
|
| Treatment protocol |
Explants from 4-day-old seedlings were cultured on B5 medium supplemented with 0.5 mg/L IBA at 28C for 12 h for the induction of lateral root formation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol (Invitrogen) and purified with the RNeasy Micro Kit (QIAGEN).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared from total RNA with the GeneChip 3' IVT Express Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization, washing, and staining procedures were conducted with Fluidics Station 450 as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
|
| Scan protocol |
Scanning procedure was conducted with GeneChip Scanner 3000 7G as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
|
| Data processing |
GCOS (MAS5.0).
|
| |
|
| Submission date |
Dec 21, 2011 |
| Last update date |
Aug 15, 2018 |
| Contact name |
Munetaka Sugiyama |
| E-mail(s) |
sugiyama@ns.bg.s.u-tokyo.ac.jp
|
| Phone |
+81-3-3814-0368
|
| Fax |
+81-3-3814-0139
|
| Organization name |
The University of Tokyo
|
| Department |
Botanical Gardens, Graduate School of Science
|
| Street address |
Hakusan 3-7-1, Bunkyo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
112-0001 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE34595 |
Transcript profile changes associated with lateral root fasciation in temperature-sensitive mutants |
|
| Relations |
| Reanalyzed by |
GSE118579 |
