|
Status |
Public on Feb 10, 2012 |
Title |
WT_root induction.12h.28C_rep2 |
Sample type |
RNA |
|
|
Source name |
WT explant
|
Organism |
Arabidopsis thaliana |
Characteristics |
strain: Landsberg erecta genotype: wild type tissue: cultured explant
|
Treatment protocol |
Explants from 4-day-old seedlings were cultured on B5 medium supplemented with 0.5 mg/L IBA at 28C for 12 h for the induction of lateral root formation.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with TRIzol (Invitrogen) and purified with the RNeasy Micro Kit (QIAGEN).
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was prepared from total RNA with the GeneChip 3' IVT Express Kit (Affymetrix).
|
|
|
Hybridization protocol |
Hybridization, washing, and staining procedures were conducted with Fluidics Station 450 as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
|
Scan protocol |
Scanning procedure was conducted with GeneChip Scanner 3000 7G as described in the GeneChip Expression Analysis Technical Manual (Affymetrix).
|
Data processing |
GCOS (MAS5.0).
|
|
|
Submission date |
Dec 21, 2011 |
Last update date |
Aug 15, 2018 |
Contact name |
Munetaka Sugiyama |
E-mail(s) |
sugiyama@ns.bg.s.u-tokyo.ac.jp
|
Phone |
+81-3-3814-0368
|
Fax |
+81-3-3814-0139
|
Organization name |
The University of Tokyo
|
Department |
Botanical Gardens, Graduate School of Science
|
Street address |
Hakusan 3-7-1, Bunkyo-ku
|
City |
Tokyo |
ZIP/Postal code |
112-0001 |
Country |
Japan |
|
|
Platform ID |
GPL198 |
Series (1) |
GSE34595 |
Transcript profile changes associated with lateral root fasciation in temperature-sensitive mutants |
|
Relations |
Reanalyzed by |
GSE118579 |