|
| Status |
Public on Apr 14, 2012 |
| Title |
Sarcoidosis whole blood microRNA SARC14 |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral blood SARC
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Male
tissue: peripheral blood
disease state: sarcoidosis
subject: SARC14
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from peripheral blood collected in PAXgene tubes.
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled with the Fluorescent Linear Amplification Kit (Agilent Technologies) following manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Quantity and labelling efficiency were verified before hybridization to human microRNA arrays (Agilent Technologies)
|
| Scan protocol |
Scanned on an Agilent Scanner G2505B. Image analysis was performed with Feature Extraction software (version 6.1.1, Agilent Technologies).
|
| Description |
SARC whole blood total microRNA
|
| Data processing |
Agilent Feature Extraction Software (v 6.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Dec 21, 2011 |
| Last update date |
Apr 14, 2012 |
| Contact name |
Jeroen Maertzdorf |
| Organization name |
MPIIB
|
| Street address |
Chariteplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL7731 |
| Series (1) |
| GSE34608 |
Gene and microRNA expression in pulmonary tuberculosis and sarcoidosis |
|
