|
| Status |
Public on Sep 01, 2012 |
| Title |
Leaf disks compatible interaction 24h |
| Sample type |
RNA |
| |
|
| Source name |
Leaf disks inoculated for 24h with M. larici-populina isolate 98AG31
|
| Organism |
Populus trichocarpa x Populus deltoides |
| Characteristics |
cultivar: 'Beaupré'
tissue: 5 cm2 leaf disks
treatment: inoculation with Melampsora larici-populina isolate 98AG31
treatment duration: 24h
|
| Biomaterial provider |
Institut National de la Recherche Agronomique (INRA Nancy, France)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction and quantity/quality control were performed as described in Rinaldi et al. 2007. RNA quality was verified by Experion StdSens capillary gels (Bio-Rad). Double-stranded (ds) cDNA were synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech, Saint-Germain-en-Laye, France), following manufacturer's instructions, with 0,5 µg of starting total RNA and 19 cycles of PCR amplification in 25µL. PCR products were purified with the QIAquick PCR Purification Kit (Qiagen, Couteboeuf, France) and quantified with a NanoDrop 1000 spectrophotometer (Labtech, Palaiseau, France).
|
| Label |
Cy3
|
| Label protocol |
Labelling by NimbleGen Company
|
| |
|
| Hybridization protocol |
Hybridization by NimbleGen Company
|
| Scan protocol |
Scanning by NimbleGen Company
|
| Data processing |
Expression values reflect across-array quantile normalization and gene calls generated using the Robust Multichip Average (RMA). For information on RMA, see Biostatistics 2003 Apr; 4(2): 249-264.
|
| |
|
| Submission date |
Dec 22, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Annegret Kohler |
| E-mail(s) |
annegret.kohler@inrae.fr
|
| Phone |
+33 (0)383 394072
|
| Organization name |
INRAE
|
| Department |
UMR 1136
|
| Lab |
Interactions Arbres/Micro-organismes
|
| Street address |
Centre INRAE Grand Est Nancy
|
| City |
Champenoux |
| ZIP/Postal code |
54280 |
| Country |
France |
| |
|
| Platform ID |
GPL13485 |
| Series (2) |
| GSE34802 |
Early infection of poplar leaves by the rust pathogen Melampsora larici-populina |
| GSE39727 |
Analysis of wound-induced transcripts in poplar leaf disks versus whole leaves |
|
