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Sample GSM852558 Query DataSets for GSM852558
Status Public on Mar 27, 2013
Title murine atherosclerotic plaque on 3 month high fat diet Replicate 3
Sample type RNA
Source name murine atherosclerotic plaque on 3 month high fat diet
Organism Mus musculus
Characteristics strain: apoe-/-
age: 6-8 weeks
Sex: Female
tissue: aortic root
Treatment protocol mice were fed on 3 month high fat diet
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol RecoverAll total nucleic acid isolation kit (Applied Biosystems) see
Label FAM
Label protocol N/A
Hybridization protocol N/A
Scan protocol N/A
Description Biological replicate 3 of 4. early atherosclerotic plaque
Data processing Taqman low density arrays were run on the 7900 HT Sequence Detection system. The ABI TaqMan SDS v2.3 software was utilized to obtain raw CT values. To review results, the raw CT data (SDS file format) were exported from the Plate Centric View into the ABI TaqMan RQ manager software. Automatic baseline and manual CTs were calculated with the RQ Manager Software for all samples. Statistical analysis: The global data set of miRs from card A and B was used for analysis. Data were calculated using Real Time StatMiner Tools [Integromics.]. Input data for analysis consisted of all samples. CT values were normalized to the most stable endogenous controls (snoRNA202, snoRNA135, snoRNA429 and MammU6 for Card A and B) according to the Genorm algorithm.
Submission date Dec 22, 2011
Last update date Mar 27, 2013
Contact name Andreas Schober
Organization name Ludwig-Maximilians-University
Department Institute for Cardiovascular Prevention
Street address Pettenkoferstr. 9
City Munich
State/province Bavaria
ZIP/Postal code 80336
Country Germany
Platform ID GPL15053
Series (2)
GSE34645 MiRNA expression profile in murine early atherosclerotic plaque compared with un-diseased arterial tissue
GSE34648 MiRNA expression profile in murine

Data table header descriptions
VALUE [delta] CT = CT,sample - CT,endogenous control.

Data table
ath-miR159a-4373390 16.337
mmu-let-7a*-4395608 8.598
mmu-let-7a-4373169 16.337
mmu-let-7b*-4395515 16.337
mmu-let-7b-4373168 4.371
mmu-let-7c-1*-4395609 16.337
mmu-let-7c-4373167 4.326
mmu-let-7d*-4378108 16.337
mmu-let-7d-4395394 6.312
mmu-let-7e-4395517 7.28
mmu-let-7f*-4395528 16.337
mmu-let-7f-4373164 16.337
mmu-let-7g*-4395622 16.337
mmu-let-7g-4395393 9.316
mmu-let-7i*-4395283 16.337
mmu-let-7i-4395332 6.296
mmu-miR-100-4373160 6.288
mmu-miR-101a*-4395637 16.337
mmu-miR-101a-4395364 10.317
mmu-miR-101b-4395661 9.381

Total number of rows: 520

Table truncated, full table size 14 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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