|
| Status |
Public on Dec 20, 2012 |
| Title |
WT-4 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA, Strain-Δ|(-2)|-7B-YUNI300 (MATa CAN1 his7-2 leu2-Δ::kanMX ura3-Δ trp1-289 ade2-1 lys2-ΔGG2899-2900) agp1:: URA3-OR1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: WT yeast
|
| Treatment protocol |
No treatment. In this study we compared a wild-type strain (WT) to a mutant strain lacking RNH201.
|
| Growth protocol |
Strains were streaked onto yeast peptone dextrose adenine (YPDA) plates and grown at 30°C for 3 days. Individual colonies were grown in 25 mL YPDA at 30°C for 6-8 hours. Cells were counted and a calculated volume from the day cultures diluted into 25 ml of YPDA and grown at 30°C overnight with constant shaking to achieve a cell density of 1-3 x 107 cells/ ml.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a RiboPure yeast kit (Ambion), as per manufacturer’s instructions.
|
| Label |
Cy3
|
| Label protocol |
Starting with 500ng of total RNA, Cy3 labeled cRNA was produced according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
For each sample, 1.65ug of Cy3 labeled cRNAs were fragmented and hybridized for 17 hours in a rotating hybridization oven.
|
| Scan protocol |
Slides were washed and then scanned with an Agilent Scanner.
|
| Description |
Gene expression at mid-log
|
| Data processing |
Data was obtained using the Agilent Feature Extraction software (v9.5), using the 1-color defaults for all parameters.
|
| |
|
| Submission date |
Dec 22, 2011 |
| Last update date |
Dec 20, 2012 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL7542 |
| Series (1) |
|
