|
| Status |
Public on Oct 28, 2024 |
| Title |
Het-Ctrl, HPC, biological rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Het-Ctrl, HPC
|
| Organism |
Mus musculus |
| Characteristics |
cell type: HPCs
genotype: Het (Polgwt/mut.)
treatment: tranduced with Ctrl vector
|
| Treatment protocol |
Six-well plates were coated with retronectin for 16 h, followed by spin at 3000 rpm, 60 min, 4°C with supernatant containing MSCV-IRES-YFP (Ctrl) or MSCV-NMyc-IRES-YFP (NMyc) secreted from GP+E86 cells. HPCs (0.5 × 106/well) from WT, Het, and Hom mice were then seeded on the plates with either Ctrl or NMyc in c-BM media.
|
| Growth protocol |
Lineage-negative BM cells (HPCs) from WT, Het, and Hom mice were separated by the autoMACS. All sorted HPCs were immediately seeded for 48 h in complete BM media (c-BM media) till transduction with NMyc or Ctrl vectors.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
DLR-labeled single-stranded DNA was synthesized using the Affymetrix GeneChip® WT PLUS Reagent Kit (Affymetrix), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Affymetrix Clariom™ S Mouse Array by GeneChip® Hybridization Oven 645 according to the protocols recommended by the manufacturer (Affymetrix, Inc).
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G (Affymetrix, Inc).
|
| Description |
HPCs isolated from heterozygous (Polgwt/mut.) mice transduced with Ctrl vector
xki025
|
| Data processing |
The data were analyzed with Affymetrix® Expression Console™ Software (Affymetrix, Inc).
|
| |
|
| Submission date |
Sep 30, 2024 |
| Last update date |
Oct 28, 2024 |
| Contact name |
Yong-Dong Wang |
| E-mail(s) |
michael.wang@stjude.org
|
| Phone |
9015954224
|
| Organization name |
St. Jude Children's Research Hospital
|
| Street address |
262 Danny Thomas Pl, MS1135
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38134 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE278440 |
Somatic mtDNA Mutation Burden Shapes Metabolic Plasticity in Leukemogenesis |
|
