|
| Status |
Public on Sep 01, 2012 |
| Title |
114B |
| Sample type |
RNA |
| |
|
| Source name |
20110929_114B
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: 114B
|
| Treatment protocol |
Yeast cells after 3hr exposure to 1 uM alpha-factor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using Qiagen RNeasy kit following manufacturer's protocol.
|
| Label |
Cy3
|
| Label protocol |
Cyanine-3 (Cy3) labeled cRNA was prepared using the Agilent One-Color Microarray-Based Gene Expression Analysis kit (Agilent) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Agilent protocol.
|
| Scan protocol |
Agilent protocol.
|
| Description |
20110929_114B
Gene expression after 3hr exposure to 1uM alpha-factor
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.1 (Agilent) using default parameters (protocol GE1-v1_91 and Grid: 012391_D_20060331) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Dec 29, 2011 |
| Last update date |
Sep 01, 2012 |
| Contact name |
Gregory Carter |
| E-mail(s) |
greg.carter@jax.org
|
| Phone |
1-207-288-6025
|
| Organization name |
The Jackson Laboratory
|
| Street address |
600 Main Street
|
| City |
Bar Harbor |
| State/province |
ME |
| ZIP/Postal code |
04609 |
| Country |
USA |
| |
|
| Platform ID |
GPL9825 |
| Series (1) |
| GSE34787 |
Use of Pleiotropy to Model Genetic Interactions in a Population |
|
