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Status
Public on Dec 01, 2005
Title
treated with N2 gas to replace air, 3 h, replicate 2
Sample type
RNA
Source name
suspension cell culture, N2 air replacement, 3 h
Organism
Arabidopsis thaliana Characteristics
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
Biomaterial provider
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
Extracted molecule
total RNA
Label
biotin
Label protocol
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type Arabidopsis suspension cell culture, pale green in colour
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Dec 01, 2005
Contact name
Rachel Clifton
E-mail(s)
crachel@cyllene.uwa.edu.au Organization name
Plant Energy Biology, ARC Centre of Excellence
Department
Biochemistry, UWA
Lab
Plant Molecular Biology Group
Street address
4th Floor CMS Building M310, University of Western Australia
City
Crawley
State/province
Western Australia
ZIP/Postal code
6009
Country
Australia
Platform ID
GPL198 Series (1)
GSE3709 Cell culture treatment study
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
308
P
0.000147
AFFX-BioB-M_at
321.4
P
0.000095
AFFX-BioB-3_at
296
P
0.00007
AFFX-BioC-5_at
807.8
P
0.00006
AFFX-BioC-3_at
495.5
P
0.000052
AFFX-BioDn-5_at
719.2
P
0.00007
AFFX-BioDn-3_at
3339
P
0.000044
AFFX-CreX-5_at
6241.6
P
0.000044
AFFX-CreX-3_at
10465.9
P
0.000052
AFFX-DapX-5_at
382.7
P
0.00007
AFFX-DapX-M_at
738.7
P
0.000052
AFFX-DapX-3_at
1104.6
P
0.000044
AFFX-LysX-5_at
45.6
P
0.023929
AFFX-LysX-M_at
82.8
P
0.002275
AFFX-LysX-3_at
91.4
P
0.000052
AFFX-PheX-5_at
85.4
P
0.001248
AFFX-PheX-M_at
109.8
P
0.00141
AFFX-PheX-3_at
154
P
0.000754
AFFX-ThrX-5_at
103.7
P
0.00011
AFFX-ThrX-M_at
158.4
P
0.00007
22810 585 Kbytes .Supplementary data files not provided
