|
| Status |
Public on Dec 01, 2005 |
| Title |
rotenone treated, 3 h, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
suspension cell culture, rotenone 3 h
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
Arabidopsis suspension cell culture, cv Lansberg erecta, 4 days post sub-culturing, sample collected 3 h post-treatment with treatment starting 2 h into the light cycle of a 16 h light 8 h dark light cycle
|
| Biomaterial provider |
Plant Molecular Biology Group: Rachel Clifton, Ryan Lister, Pia Sappl
|
| Treatment protocol |
250 mL flasks of suspension cells were treated by the addition of 480 ul of 10 mM rotenone to obtain a final concentration of 40 uM in the flask, samples were collected 3 h after treatment.
|
| Extracted molecule |
total RNA |
| Label |
biotin
|
| Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Wild type Arabidopsis suspension cell culture, pale green in colour
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Dec 01, 2005 |
| Last update date |
Dec 01, 2005 |
| Contact name |
Rachel Clifton |
| E-mail(s) |
crachel@cyllene.uwa.edu.au
|
| Organization name |
Plant Energy Biology, ARC Centre of Excellence
|
| Department |
Biochemistry, UWA
|
| Lab |
Plant Molecular Biology Group
|
| Street address |
4th Floor CMS Building M310, University of Western Australia
|
| City |
Crawley |
| State/province |
Western Australia |
| ZIP/Postal code |
6009 |
| Country |
Australia |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE3709 |
Cell culture treatment study |
|
