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Status
Public on Mar 31, 2006
Title
bhh treated 3
Sample type
RNA
Source name
pronephric explant
Organism
Xenopus laevis Characteristics
Wild type, Stage 24 pronephric explant
Extracted molecule
total RNA
Extraction protocol
Pronephric region explants were prepared from stage 24 embryos using a gastromaster (Xenotek) with the tip set to a width of 70 microns. Rectangular explants, approximately 70 microns (dorsal to ventral) x 100 microns (anteriorposterior) and 70 microns (laterally) were cut from the flanks of both sides of embryos. 300 explants were extracted with Trizol (Gibco)
Label
biotin
Label protocol
1 µg of total RNA was processed using MessageAmp™ aRNA Amplification Kit (AMBION) to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
St 24 bhh treated pronephric explants
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Dec 01, 2005
Last update date
Jan 17, 2006
Contact name
Curtis R Altmann
E-mail(s)
craltmann@gmail.com Phone
8506442925
Organization name
Goodwin Procter LLP
Street address
599 Lexington Avenue
City
New York
State/province
NY
ZIP/Postal code
10022
Country
USA
Platform ID
GPL1318 Series (1)
GSE3712 Bhh signaling in pronephric development
Data table header descriptions ID_REF VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE AFFX-BioB-5_at
187.3
P
0.000509
AFFX-BioB-M_at
286.7
P
0.000052
AFFX-BioB-3_at
144.1
P
0.000095
AFFX-BioC-5_at
615.3
P
0.000052
AFFX-BioC-3_at
536.1
P
0.000044
AFFX-BioDn-5_at
973.9
P
0.000044
AFFX-BioDn-3_at
2009.9
P
0.000127
AFFX-CreX-5_at
5606.4
P
0.000044
AFFX-CreX-3_at
7908
P
0.000044
AFFX-DapX-5_at
15.5
A
0.262827
AFFX-DapX-M_at
19.7
A
0.300606
AFFX-DapX-3_at
5.9
A
0.891021
AFFX-LysX-5_at
6.8
A
0.617401
AFFX-LysX-M_at
4.2
A
0.672921
AFFX-LysX-3_at
4.7
A
0.574038
AFFX-PheX-5_at
2.5
A
0.876428
AFFX-PheX-M_at
1.1
A
0.910522
AFFX-PheX-3_at
3.9
A
0.891021
AFFX-ThrX-5_at
16.5
A
0.724854
AFFX-ThrX-M_at
12.9
A
0.455413
15611 504 Kbytes .Supplementary data files not provided
