|
| Status |
Public on Jan 07, 2012 |
| Title |
Intestine_KO_day8_1 [gene-level] |
| Sample type |
RNA |
| |
|
| Source name |
small intestine (jejunum)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B/6
genotype/variation: Dicer KO
age: 8 weeks
|
| Treatment protocol |
Tamoxifen gavage to induce conditional knockout
|
| Growth protocol |
Conditional knockout of Dicer exon 21 & 22 in C57B/6 mice of 8 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Human Exon 1.0 ST Arrays (Affymetrix) and scanned at the Johns Hopkins Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
THua-DIC-Cre_TA_1014-1a-mmExon1_(MoEx-1_0-st-v1).CEL
|
| Data processing |
affymetrix-algorithm-name = rma-gene-extended
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.1.2800.19935
program-company = Affymetrix
The statistical analysis was performed using GeneSpring GX 10.
|
| |
|
| Submission date |
Jan 06, 2012 |
| Last update date |
Jan 07, 2012 |
| Contact name |
Tai-Chung Huang |
| E-mail(s) |
huang@jhmi.edu
|
| Phone |
410-502-6665
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Institute of Genetic Medicine
|
| Lab |
Akhilesh Pandey
|
| Street address |
733 N Broadway BRB R560
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6096 |
| Series (2) |
| GSE34905 |
Differential gene expression caused by Dicer knockout in small intestine |
| GSE34910 |
Differential expression caused by Dicer knockout in small intestine |
|
| Relations |
| Alternative to |
GSM857376 (exon-level analysis) |
