|
| Status |
Public on Jan 07, 2012 |
| Title |
Intestine_KO_day8_1 |
| Sample type |
RNA |
| |
|
| Source name |
small intestine (jejunum)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B/6
genotype/variation: Dicer KO
age: 8 weeks
|
| Treatment protocol |
Tamoxifen gavage to induce conditional knockout
|
| Growth protocol |
Conditional knockout of Dicer exon 21 & 22 in C57B/6 mice of 8 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini (Qiagen)
|
| Label |
biotin
|
| Label protocol |
FlashTag Biotin HSR RNA labeling kit
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip miRNA array (Affymetrix) and scanned at the Johns Hopkins Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
Our data was processed with "Affymetrix miRNA QC Tool version 1.0", within which background subtraction and quantile normalization were done. The summarized probe set data was log2 transformed. The statistical analysis was performed using GeneSpring GX 10.
|
| |
|
| Submission date |
Jan 06, 2012 |
| Last update date |
Jan 07, 2012 |
| Contact name |
Tai-Chung Huang |
| E-mail(s) |
huang@jhmi.edu
|
| Phone |
410-502-6665
|
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Institute of Genetic Medicine
|
| Lab |
Akhilesh Pandey
|
| Street address |
733 N Broadway BRB R560
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE34909 |
Differential miRNA expression caused by Dicer knockout in small intestine |
| GSE34910 |
Differential expression caused by Dicer knockout in small intestine |
|
