|
| Status |
Public on Oct 25, 2024 |
| Title |
NFE2L1DC-2 |
| Sample type |
SRA |
| |
|
| Source name |
HepG2 cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2 cell line
genotype: NFE2L1DC overexpression
|
| Growth protocol |
The experimental cells were cultured in high glucose DMEM containing 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After cells were lysed with Trizol, they were shipped to Sangon Biotech (Shanghai, China) in dry ice.
Total RNA transcriptomic sequencing of HepG2-pLVX, HepG2-NFE2L1, and HepG2-NFE2L1ΔC cells was performed by Sangon Biotech (Shanghai, China) on the HiseqNOVA platform (contract No.: MRNA193522ZZ).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
The raw image data files obtained by Illumina Hiseq are converted into raw sequencing sequences (Sequenced Reads) through CASAVA base calling analysis, which we refer to as Raw Data or Raw Reads. The results are stored in FASTQ (abbreviated as fq) file format, which includes the sequence information of the sequencing sequences (reads) and their corresponding sequencing quality information.
Assembly: Homo sapiens genome GRCh38
|
| |
|
| Submission date |
Oct 24, 2024 |
| Last update date |
Oct 25, 2024 |
| Contact name |
Lu Qiu |
| E-mail(s) |
qiulu@zzu.edu.cn
|
| Phone |
13140169101
|
| Organization name |
The First Affiliated Hospital of Zhengzhou University
|
| Street address |
No.1 East Jianshe Road, Erqi District
|
| City |
Zhengzhou |
| State/province |
Henan |
| ZIP/Postal code |
450052 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE280238 |
Discovery and analysis of NFE2L1's non-transcriptional activity by transcriptome sequencing in hepatocellular carcinoma HepG2 cells |
|
| Relations |
| BioSample |
SAMN44421808 |
| SRA |
SRX26487654 |
