|
| Status |
Public on Jul 01, 2014 |
| Title |
Blood SCD patientA0005 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
age (years): 5.8
clinical status: E
severity score: 0.404
Sex: F
hb phenotype: SS
white blood cells: 17
mean corpuscular volume: 87.1
red blood cells: 3
genotypic pc1: -0.0504
genotypic pc2: 0.0295
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from whole blood collected in Tempus tubes using the Tempus Spin RNA Isolation kit (Applied Biosystems). Total RNA was then globin-mRNA-depleted using GlobinClear kit (Ambion). Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Sample1
globin mRNA-depleted total RNA
|
| Data processing |
The data was log2 transfromed and normalised using quantile normalisation with JMP Genomics
|
| |
|
| Submission date |
Jan 11, 2012 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Jacklyn Quinlan |
| E-mail(s) |
jacki.quinlan@gmail.com
|
| Phone |
514-345-4931
|
| Organization name |
CHU Ste-Justine Hospital
|
| Department |
Pediatrics
|
| Lab |
Philip Awadalla
|
| Street address |
3175 Cote Ste-Catherine
|
| City |
Montreal |
| State/province |
Qc |
| ZIP/Postal code |
H3T 1C5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE35007 |
The genomic architecture of sickle cell disease in children from West Africa |
|
