|
Status |
Public on Jul 01, 2012 |
Title |
Steer ID# 410 Control diet, subcutaneous fat |
Sample type |
RNA |
|
|
Source name |
Subcutaneous fat (backfat), Control diet
|
Organism |
Bos taurus |
Characteristics |
diet: Control diet tissue: Subcutaneous fat
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction was performed by homogenizing the fat tissue samples with TRIZOL® (TRI reagent, Invitrogen, Carlsbad, CA, USA) following the manufactures instructions for samples with high fat content.
|
Label |
Cy3
|
Label protocol |
Total RNA (100 ng/sample) was firstly labeled with the AGILENT miRNA Complete Labeling and Hyb Kit (Version 2.1) by dephosphorylation with Calf Intestinal Phosphatase followed by denaturing and ligation with Cyanine3-pCp to the 3’ end.
|
|
|
Hybridization protocol |
The labeled RNA was hybridized with array slides with hybridization buffer and 10X GE blocking agent, and were incubated at 55ºC for ~20 hours. Finally, the arrays were washed with GE wash buffers.
|
Scan protocol |
The arrays were scanned at 5 μM resolution on an Agilent G2565CA High Resolution Scanner (Agilent Technologies). Data was processed through Agilent’s Feature Extraction software version 10.7.3.1 using the protocol miRNA_107_Sep09
|
Description |
Sample name: 410BF Raw data file: 253020810002_201010151644_S01_miRNA_107_Sep09_2_4.txt
|
Data processing |
The data was normalized to the 75th percentile using GeneSpring GX 11.5 (Agilent Technologies)
|
|
|
Submission date |
Jan 11, 2012 |
Last update date |
Jul 01, 2012 |
Contact name |
Josue Moura Romao |
Organization name |
University of Alberta
|
Department |
AFNS
|
Lab |
AGP
|
Street address |
116 St. and 85 Ave.
|
City |
Edmonton |
State/province |
Alberta |
ZIP/Postal code |
T6G 2P5 |
Country |
Canada |
|
|
Platform ID |
GPL15110 |
Series (1) |
GSE35012 |
MicroRNA expression in subcutaneous and visceral fat of beef steers fed different high or low fat diets |
|