|
| Status |
Public on Jul 01, 2012 |
| Title |
Steer ID# 302 High fat diet, subcutaneous fat |
| Sample type |
RNA |
| |
|
| Source name |
Subcutaneous fat (backfat), High fat diet
|
| Organism |
Bos taurus |
| Characteristics |
diet: High fat diet
tissue: Subcutaneous fat
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed by homogenizing the fat tissue samples with TRIZOL® (TRI reagent, Invitrogen, Carlsbad, CA, USA) following the manufactures instructions for samples with high fat content.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng/sample) was firstly labeled with the AGILENT miRNA Complete Labeling and Hyb Kit (Version 2.1) by dephosphorylation with Calf Intestinal Phosphatase followed by denaturing and ligation with Cyanine3-pCp to the 3’ end.
|
| |
|
| Hybridization protocol |
The labeled RNA was hybridized with array slides with hybridization buffer and 10X GE blocking agent, and were incubated at 55ºC for ~20 hours. Finally, the arrays were washed with GE wash buffers.
|
| Scan protocol |
The arrays were scanned at 5 μM resolution on an Agilent G2565CA High Resolution Scanner (Agilent Technologies). Data was processed through Agilent’s Feature Extraction software version 10.7.3.1 using the protocol miRNA_107_Sep09
|
| Description |
Sample name: 302BF
Raw data file:
253020810004_201012091611_S01_miRNA_107_Sep09_2_1.txt
|
| Data processing |
The data was normalized to the 75th percentile using GeneSpring GX 11.5 (Agilent Technologies)
|
| |
|
| Submission date |
Jan 11, 2012 |
| Last update date |
Jul 01, 2012 |
| Contact name |
Josue Moura Romao |
| Organization name |
University of Alberta
|
| Department |
AFNS
|
| Lab |
AGP
|
| Street address |
116 St. and 85 Ave.
|
| City |
Edmonton |
| State/province |
Alberta |
| ZIP/Postal code |
T6G 2P5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL15110 |
| Series (1) |
| GSE35012 |
MicroRNA expression in subcutaneous and visceral fat of beef steers fed different high or low fat diets |
|
