|
| Status |
Public on May 06, 2013 |
| Title |
N2 flow control t5 replicates b_2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
plasmat5b
|
| Organism |
Bacillus cereus |
| Characteristics |
strain: ATCC 14579
exposure time: plasma treated exposure 6X5 min
|
| Treatment protocol |
The vegetative cells were tranferred onto a celulose nitrate filter by filtration and the filters were placed ontop of nutrient agar plates. The plates, divided into 6 sectors, were exposed to a nitrogen gas assisted low temperature plasma at atmospheric pressure for 0, 1, 2, or 5 minutes per sector.
|
| Growth protocol |
Bacillus cereus ATCC 14579 (type strain) was grown in BHI at 30C, 200 rpm, to OD 0.5 (mid exponential phase)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The filters were tranferred to tubes containing Tri-reagent, further total RNA extraction was performed as previously described (Mols et al., 2010).
|
| Label |
cy3
|
| Label protocol |
cDNA synthesis, labelling, microarray hybridization and scanning was performed as previously described (Mols et al., 2010).
|
| |
|
| Channel 2 |
| Source name |
N2t5b
|
| Organism |
Bacillus cereus |
| Characteristics |
strain: ATCC 14579
exposure time: nitrogen flow treated exposure 6X5 min
|
| Treatment protocol |
The vegetative cells were tranferred onto a celulose nitrate filter by filtration and the filters were placed ontop of nutrient agar plates. The plates, divided into 6 sectors, were exposed to a nitrogen gas assisted low temperature plasma at atmospheric pressure for 0, 1, 2, or 5 minutes per sector.
|
| Growth protocol |
Bacillus cereus ATCC 14579 (type strain) was grown in BHI at 30C, 200 rpm, to OD 0.5 (mid exponential phase)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The filters were tranferred to tubes containing Tri-reagent, further total RNA extraction was performed as previously described (Mols et al., 2010).
|
| Label |
cy5
|
| Label protocol |
cDNA synthesis, labelling, microarray hybridization and scanning was performed as previously described (Mols et al., 2010).
|
| |
|
| |
| Hybridization protocol |
cDNA synthesis, labelling, microarray hybridization and scanning was performed as previously described (Mols et al., 2010).
|
| Scan protocol |
Scanned on an Agilent G2565BA scanner.
|
| Description |
US22502548_251734310045_S01_Bcereus14579_3rdDesign_2_4
plasma vs N2 flow t5
|
| Data processing |
Agilent Feature Extraction Software (v 8.1.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Jan 12, 2012 |
| Last update date |
May 07, 2013 |
| Contact name |
Maarten Mols |
| Organization name |
University of Groningen
|
| Street address |
Nijenborgh 7
|
| City |
Groningen |
| ZIP/Postal code |
9747 AG |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9493 |
| Series (1) |
| GSE35060 |
Physiological and transcriptional response of Bacillus cereus treated with low-temperature nitrogen gas plasma |
|
