|
| Status |
Public on Feb 01, 2012 |
| Title |
Cardiomyocytes derived from normal iPSC treated by Serca2a, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Normal iPSC treated by Serca2a
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Cardiomyocytes (derived from normal iPSC)
genotype/variation: Normal
gene therapy: Serca2a
|
| Treatment protocol |
iPSC were treated by Adenovirus-Serca2a
|
| Growth protocol |
iPSCs were maitained with mTESR-1 medium on matrigel coated surfaces. iPSC-derived cardiomyocytes were maitained with DMEM plus 10% FBS in tissue culture dishes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization were followed standard Affymetrix protocol in Affymetrix GeneChip Hybridization Oven 640
|
| Scan protocol |
GeneChips were scanned using theAffymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from iPSC
Control-Serca2a-2
Control-Serca2a-2.CEL
|
| Data processing |
The data were analyzed with Affymetrix Expression Console using Affymetrix default analysis settings and RMA as normalization method.
|
| |
|
| Submission date |
Jan 14, 2012 |
| Last update date |
Feb 01, 2012 |
| Contact name |
Leng Han |
| E-mail(s) |
hanleng0926@hotmail.com
|
| Organization name |
Stanford University
|
| Street address |
265 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE35108 |
Expression data from patient iPSC and iPSC-derived cardiomyocytes |
|
