|
| Status |
Public on Feb 08, 2006 |
| Title |
Invitrogen yeast protoarray probed with YFL010C WW domain, first experiment |
| Sample type |
protein |
| |
|
| Source name |
E.coli overexpression strain
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
biotinylated WW domain fusion protein
|
| Extracted molecule |
protein |
| Extraction protocol |
WW domain probes were purified as fusion proteins from E. coli overexpression strains
|
| Label |
Alexa Fluor 647
|
| |
|
| Hybridization protocol |
Biotinylated fusion protein probes were applied at a concentration of 50 uM using conditions supplied by Invitrogen. Interactions were visulalized by the application of Alexa Fluor 647-streptavavidin
|
| Scan protocol |
Fluorescent array images were collected for Cy5 with a GenePix 4000A fluorescent scanner and image intensity data were extracted and analyzed with GenePix Pro 3.0 analysis software.
|
| Description |
WW domain GST-fusion protein expressed and purified from E. coli, and chemically biotinylated with Pierce NHS-LC-LC-biotin
|
| Data processing |
Each protein is printed in duplicate on the array; for each protein, the values of F635_MEAN - B635_MEAN were averaged (VALUE). The arrays are printed in a total of 48 blocks. The mean and standard deviation of signals from each block were calculated. If a protein's VALUE was greater than three standard deviations above the mean for it's parent block, it was called significant.
|
| |
|
| Submission date |
Dec 06, 2005 |
| Last update date |
Feb 08, 2006 |
| Contact name |
Jay R. Hesselberth |
| E-mail(s) |
jay.hesselberth@cuanschutz.edu
|
| Organization name |
University of Colorado School of Medicine
|
| Department |
Biochemistry and Molecular Gentetics
|
| Lab |
Jay Hesselberth
|
| Street address |
12801 E 17TH AVE
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platform ID |
GPL3234 |
| Series (1) |
| GSE3758 |
Interactions between Saccharomyces cerevisiae WW domains and proteins discovered using protein microarrays |
|
