|
| Status |
Public on Aug 14, 2012 |
| Title |
tumour#10 gene expression |
| Sample type |
RNA |
| |
|
| Source name |
tumour
|
| Organism |
Mus musculus |
| Characteristics |
cell type: B cell lymphoma
tumor number: 10
|
| Treatment protocol |
Diagnostic sorted B cell lymphoma samples derived from Cgamma1-cre; R26StopFLMYC;R26StopFLP110* transplanted animals
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was purified with Qiagen RNeasy Mini kits (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
For each sample preparation, total RNA was converted into double-stranded cDNA by reverse transcription using the 3' IVT Express Kit from Affymetrix according to the manufacturer's recommendations.
|
| |
|
| Hybridization protocol |
Hybridization, washing, and staining protocols, respectively, were performed on Affymetrix GeneChip instruments (Hybridization Oven 640, Fluidics Station FS450) as recommended by the manufacturer.
|
| Scan protocol |
Scanning was performed on Affymetrix GeneChip Scanner GCS3000 instruments as recommended by the manufacturer using default settings. The software used was GCOS 1.2 or higher.
|
| Description |
Gene expression data from murine B cell lymphoma
|
| Data processing |
The data were analyzed with BRB ArrayTools using RMA. Intensity values were log2 transformed
|
| |
|
| Submission date |
Jan 19, 2012 |
| Last update date |
Aug 14, 2012 |
| Contact name |
Lars Bullinger |
| E-mail(s) |
lars.bullinger@charite.de
|
| Phone |
+49-30-450-553111
|
| Organization name |
Charité
|
| Department |
Hematology, Oncology and Tumorimmunology
|
| Street address |
Augustenburger Platz 1
|
| City |
Berlin |
| ZIP/Postal code |
13353 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE35219 |
Synergy between PI3K Signaling and MYC in Burkitt Lymphomagenesis |
|
