|
| Status |
Public on Jan 25, 2012 |
| Title |
C4-2B Isoflavone 72h |
| Sample type |
RNA |
| |
|
| Source name |
C4-2B cells treated with 25uM isoflavone for 72 hours
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: C4-2B prostate cancer cells
agent: isoflavone
time: 72 hours
|
| Treatment protocol |
Cells were treated with 25uM B-DIM or 25uM isoflavone (the concentrations of isoflavone we described refer to the concentration of genistein in isoflavone mixture).
|
| Growth protocol |
C4-2B cells were cultured in RPMI 1640 medium supplemented with 10% FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol reagent followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using Affymetrix standard protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol was used.
|
| Scan protocol |
The arrays were scanned using standard Affymetrix scanning protocol.
|
| Description |
gene expression data from isoflavone treated C4-2B cells
FS22-C4_S_72.CEL
|
| Data processing |
The data were analyzed with Microarray Suite using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 25, 2012 |
| Last update date |
Jan 25, 2012 |
| Contact name |
Fazlul H. Sarkar |
| E-mail(s) |
fsarkar@med.wayne.edu
|
| Phone |
313-576-8327
|
| Organization name |
Wayne State University School of Medicine
|
| Department |
Pathology
|
| Street address |
4100 John R
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE35324 |
Genome-wide analysis of gene expression in isoflavone and 3,3’-diindolylmethane treated C4-2B prostate cancer cells |
|
