|
| Status |
Public on Apr 08, 2006 |
| Title |
Mutant Histone H2B^3-32 replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Yeast RNA
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
PY016 (mutant Histone H2B^3-32)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were grown to mid-log phase (OD600=0.5-0.7) in rich media (YPD). Cells were lysed and RNA was extracted by hot acid phenol chloroform and phenol chloroform isoamyl alcohol extraction.
|
| Label |
biotin
|
| Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Mutant yeast strain Histone H2B^3-32
|
| Data processing |
Affymetrix Microarray Suite version 5.0
|
| |
|
| Submission date |
Dec 08, 2005 |
| Last update date |
May 21, 2012 |
| Contact name |
John J. Wyrick |
| E-mail(s) |
jwyrick@vetmed.wsu.edu
|
| Phone |
509-335-8785
|
| Organization name |
Washington State University
|
| Department |
School of Molecular Biosciences
|
| Street address |
Biotechnology Life Sciences 241
|
| City |
Pullman |
| State/province |
WA |
| ZIP/Postal code |
99164 |
| Country |
USA |
| |
|
| Platform ID |
GPL90 |
| Series (2) |
| GSE3802 |
Histone H2B^3-32 |
| GSE3806 |
Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations |
|
