|
Status |
Public on Apr 08, 2006 |
Title |
Mutant Histone H2B^30-37 replicate 3 |
Sample type |
RNA |
|
|
Source name |
Yeast RNA
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
PY020 (mutant Histone H2B^30-37)
|
Extracted molecule |
total RNA |
Extraction protocol |
Cells were grown to mid-log phase (OD600=0.5-0.7) in rich media (YPD). Cells were lysed and RNA was extracted by hot acid phenol chloroform and phenol chloroform isoamyl alcohol extraction.
|
Label |
biotin
|
Label protocol |
Approximately 20 µg of total RNA was processed to produce biotinylated cRNA targets.
|
|
|
Hybridization protocol |
standard Affymetrix procedures
|
Scan protocol |
standard Affymetrix procedures
|
Description |
Mutant yeast strain Histone H2B^30-37
|
Data processing |
Affymetrix Microarray Suite version 5.0
|
|
|
Submission date |
Dec 08, 2005 |
Last update date |
May 21, 2012 |
Contact name |
John J. Wyrick |
E-mail(s) |
jwyrick@vetmed.wsu.edu
|
Phone |
509-335-8785
|
Organization name |
Washington State University
|
Department |
School of Molecular Biosciences
|
Street address |
Biotechnology Life Sciences 241
|
City |
Pullman |
State/province |
WA |
ZIP/Postal code |
99164 |
Country |
USA |
|
|
Platform ID |
GPL90 |
Series (2) |
GSE3805 |
Histone H2B^30-37 |
GSE3806 |
Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations |
|