|
| Status |
Public on Sep 29, 2012 |
| Title |
IL-4 treated macrophages-3 |
| Sample type |
RNA |
| |
|
| Source name |
monocyte derived macrophages
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: Random Healthy donors
cell type: macrophages
treatment: 48 hours with 20 ng/ml of IL-4
replicate: Donor 3
|
| Treatment protocol |
At day 7 cells were treated with IL-4 for 48 hours with 20 ng/ml of IL-4.
|
| Growth protocol |
Human ciculating monocytes were isolated using a double gradient of Ficall and Percoll, followed by an additional step with the monocyte isolation Kit II of Miltenyi. Cells were cultured in tissue culture plastic for 7 days in X-Viv0 10 medium supplemented with 1% autologous serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicates: Donor 3
|
| Data processing |
The data were normalised using quantile normalisation in Lumi (R Bioconductor).
|
| |
|
| Submission date |
Jan 30, 2012 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6947 |
| Series (2) |
| GSE35433 |
Genome-wide analysis of human macrophages stimulated with IL-4 (20ng/ml) (Illumina) |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
|
