|
| Status |
Public on Sep 29, 2012 |
| Title |
Biogel M2 |
| Sample type |
RNA |
| |
|
| Source name |
biogel-elicited murine peritoneal macrophages, incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
replicate: 3
cell type: biogel-elicited murine peritoneal macrophages
treatment: incubated in OptiMEM-10 + IL-4 (supernatant from IL-4 producing cell line) for 24 hrs on tissue culture plastic
|
| Treatment protocol |
Cells were treated for 24 hours with IL-4 (supernatant of mIL-4 cDNA transfected X63Ag8-653 plasmcytoma cell line).
|
| Growth protocol |
Mouse thioglycollate and Biogel elicited macrophages were isolated by adhesion and cultured on Pemanox or Tissue culture plastic, in RPMI 1640 .
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
Replicate 3
|
| Data processing |
RMA in Affy R bioconductor
|
| |
|
| Submission date |
Jan 30, 2012 |
| Last update date |
Apr 12, 2018 |
| Contact name |
Fernando O Martinez Estrada |
| E-mail(s) |
f.martinezestrada@surrey.ac.uk
|
| Organization name |
University of Surrey
|
| Department |
Faculty of Health & Medical Sciences
|
| Lab |
Innate Immunity
|
| Street address |
Stag Hill
|
| City |
Guildford |
| State/province |
Surrey |
| ZIP/Postal code |
GU2 7XH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE35436 |
Genome-wide analysis of mouse macrophages stimulated with IL-4 (Biogel and thioglycollate macrophages) (Affymetrix) |
| GSE35495 |
Genome-wide analysis of human and mouse macrophages, resting and activated |
|
