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Sample GSM871646 Query DataSets for GSM871646
Status Public on Aug 24, 2012
Title U10_09_male
Sample type SRA
 
Source name male_small flower buds
Organism Silene latifolia
Characteristics population: 10 generation inbred line
tissue: small flower buds
gender: male
Extracted molecule total RNA
Extraction protocol DNAse treatment; Transcripts purification;Transcripts breaking in presence of Zn; Double-stranded cDNA synthesis using random primers and RNase H; Ends repair; 3' A addition; Ligation of adapters; Gel purification to isolate fragments of specified inserts size; PCR amplification to generate the DNA Colonies Template Library, library purification;Quality control of the DNA Colony Template Library by cloning an aliquot into a TOPO plasmid and capillary sequencing of 4-8 clones;Library quantification and dilution at 10 nM; Flow-cell preparation on the cBot; High-throughput DNA sequencing on the illumina HiSeq 2000; Real-time base-calling on the HiSeq computer.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
 
Description U10_9
Data processing none
 
Submission date Feb 07, 2012
Last update date May 15, 2019
Contact name Niklaus Zemp
E-mail(s) niklaus.zemp@env.ethz.ch
Organization name ETH Zurich
Department IBZ
Street address Universitätsstrasse 16
City Zürich
ZIP/Postal code 8092
Country Switzerland
 
Platform ID GPL15196
Series (1)
GSE35563 Rapid de novo evolution of X chromosome dosage compensation in Silene latifolia, a plant with young sex chromosomes
Relations
SRA SRX118779
BioSample SAMN00783717

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data not provided for this record

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