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| Status |
Public on Aug 24, 2012 |
| Title |
U10_39_female |
| Sample type |
SRA |
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| Source name |
female_small flower buds
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| Organism |
Silene latifolia |
| Characteristics |
population: 10 generation inbred line
tissue: small flower buds
gender: female
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| Extracted molecule |
total RNA |
| Extraction protocol |
DNAse treatment; Transcripts purification;Transcripts breaking in presence of Zn; Double-stranded cDNA synthesis using random primers and RNase H; Ends repair; 3' A addition; Ligation of adapters; Gel purification to isolate fragments of specified inserts size; PCR amplification to generate the DNA Colonies Template Library, library purification;Quality control of the DNA Colony Template Library by cloning an aliquot into a TOPO plasmid and capillary sequencing of 4-8 clones;Library quantification and dilution at 10 nM; Flow-cell preparation on the cBot; High-throughput DNA sequencing on the illumina HiSeq 2000; Real-time base-calling on the HiSeq computer.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
U10_39
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| Data processing |
none
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| Submission date |
Feb 07, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Niklaus Zemp |
| E-mail(s) |
niklaus.zemp@env.ethz.ch
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| Organization name |
ETH Zurich
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| Department |
IBZ
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| Street address |
Universitätsstrasse 16
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| City |
Zürich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
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| Platform ID |
GPL15196 |
| Series (1) |
| GSE35563 |
Rapid de novo evolution of X chromosome dosage compensation in Silene latifolia, a plant with young sex chromosomes |
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| Relations |
| SRA |
SRX118782 |
| BioSample |
SAMN00783720 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data not provided for this record |
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